IRE1 alpha KA-pcDNA3.EGFP
(Plasmid #13010)

• Depositing Lab: Fumihiko Urano
• Publication: Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Backbone

• Vector backbone: pcDNA3-EGFP
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inositol Requiring Enzyme-1
• Alt name: IRE1
• Species: H. sapiens (human)
• Insert Size (bp): 3500
• Mutation: R4L, K599A
• Entrez Gene: ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA was provided by Dr. Randy Kaufman at the University of Michigan. The vector was provided by Dr. David Ron at the New York Univeristy School of Medicine.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Michigan-IRE1
• Industry Terms:
  • Not Available to Industry