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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Myc-His(-)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman sulfatase 1 delta CC
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Alt nameHSulf-1 delta CC
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Alt nameHSulf-1
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SpeciesH. sapiens (human)
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Insert Size (bp)2600
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Mutationchanged Cysteine 87 to Alanine
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Entrez GeneSULF1 (a.k.a. SULF-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC was a gift from Steven Rosen (Addgene plasmid # 13005 ; http://n2t.net/addgene:13005 ; RRID:Addgene_13005) -
For your References section:
Cloning and characterization of two extracellular heparin-degrading endosulfatases in mice and humans. Morimoto-Tomita M, Uchimura K, Werb Z, Hemmerich S, Rosen SD. J Biol Chem. 2002 Dec 20. 277(51):49175-85. 10.1074/jbc.M205131200 PubMed 12368295