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Addgene

pcDNA3.1/Myc-His(-)-HSulf-1
(Plasmid #13003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Myc-His(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human sulfatase 1
  • Alt name
    HSulf-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • Entrez Gene
    SULF1 (a.k.a. SULF-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/Myc-His(-)-HSulf-1 was a gift from Steven Rosen (Addgene plasmid # 13003 ; http://n2t.net/addgene:13003 ; RRID:Addgene_13003)

  • For your References section:

    Cloning and characterization of two extracellular heparin-degrading endosulfatases in mice and humans. Morimoto-Tomita M, Uchimura K, Werb Z, Hemmerich S, Rosen SD. J Biol Chem. 2002 Dec 20. 277(51):49175-85. 10.1074/jbc.M205131200 PubMed 12368295
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