Skip to main content
Addgene

pBS mTSP-4
(Plasmid #12997)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSP-4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Thbs4 (a.k.a. TS, TSP, TSP4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes the region from an EcoRI site in the third type 2 repeat to the 3' end.

This vector contains a fragment of the TSP-4 gene, as noted in the associated manuscript by Newton et al

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS mTSP-4 was a gift from Jack Lawler (Addgene plasmid # 12997 ; http://n2t.net/addgene:12997 ; RRID:Addgene_12997)

  • For your References section:

    The thrombospondin-4 gene. Newton G, Weremowicz S, Morton CC, Jenkins NA, Gilbert DJ, Copeland NG, Lawler J. Mamm Genome. 1999 Oct . 10(10):1010-6. 10.1007/s003359901149 PubMed 10501972
Related items:
Commonly requested with: