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Addgene

pGEM2 hTSP-1
(Plasmid #12993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSP-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • GenBank ID
    X04665.1 CAA28370.1
  • Entrez Gene
    THBS1 (a.k.a. THBS, THBS-1, TSP, TSP-1, TSP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes complete coding region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM2 hTSP-1 was a gift from Jack Lawler (Addgene plasmid # 12993 ; http://n2t.net/addgene:12993 ; RRID:Addgene_12993)
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