pFLIPglu-2uDelta13
(Plasmid #12990)

• Depositing Lab: Wolf Frommer
• Publication: Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Backbone

• Vector backbone: pRT100/pPZP312
• Vector type: Plant Expression ; Plant Nanosensor
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FLIPglu F16F D152A Delta13
• Alt name: ECFP-mglB-EYFP
• Alt name: Periplasmic glucose binding protein
• Species: E. coli
• Insert Size (bp): 2280
• Mutation: D152A mutation in binding protein
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCR of MglB
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html