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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-EGFP
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRac1
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SpeciesH. sapiens (human)
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Insert Size (bp)579
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GenBank IDAF498964
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 for EGFP
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-EGFP-Rac1-wt was a gift from Gary Bokoch (Addgene plasmid # 12980 ; http://n2t.net/addgene:12980 ; RRID:Addgene_12980) -
For your References section:
Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu K, Bokoch GM, Hahn KM. J Biol Chem. 2000 Mar 31. 275(13):9725-33. 10.1074/jbc.275.13.9725 PubMed 10734125
Map uploaded by the depositor.
