Bxb1Donor_G102H_ADRB2_LucReporter
(Plasmid #129794)

• Purpose: encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
• Depositing Lab: Sriram Kosuri
• Publication: Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: Genewiz
• Backbone size w/o insert (bp): 6350
• Total vector size (bp): 7660
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ADRB2
• Alt name: Beta-2 Adrenergic Receptor
• Species: H. sapiens (human)
• Insert Size (bp): 1314
• Mutation: G102H
• GenBank ID: 154
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: N/A
• Tag / Fusion Protein:
  • 3x FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ttgtcgacgacggcggtct
• 3′ sequencing primer: GCACACCGGCCTTATTCC

Resource Information

• Supplemental Documents:
  • bxb1donor_g102h_adrb2_lucreporter.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry