AmiE_I122L_pAG
(Plasmid
#129792)
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PurposeConstitutive expression plasmid used in the growth selections of AmiE I122L.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG
- Backbone size w/o insert (bp) 3583
- Total vector size (bp) 4612
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAmiE I122L
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Insert Size (bp)1029
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AmiE_I122L_pAG was a gift from Timothy Whitehead (Addgene plasmid # 129792 ; http://n2t.net/addgene:129792 ; RRID:Addgene_129792) -
For your References section:
Impact of In Vivo Protein Folding Probability on Local Fitness Landscapes. Faber MS, Wrenbeck EE, Azouz LR, Steiner PJ, Whitehead TA. Mol Biol Evol. 2019 Dec 1;36(12):2764-2777. doi: 10.1093/molbev/msz184. 10.1093/molbev/msz184 PubMed 31400199