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Bxb1Donor_W99A_ADRB2_LucReporter
(Plasmid #129789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genewiz
  • Backbone size w/o insert (bp) 6350
  • Total vector size (bp) 7660
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADRB2
  • Alt name
    Beta-2 Adrenergic Receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1314
  • Mutation
    W99A
  • GenBank ID
    154
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
  • Promoter N/A
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttgtcgacgacggcggtct
  • 3′ sequencing primer GCACACCGGCCTTATTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bxb1Donor_W99A_ADRB2_LucReporter was a gift from Sriram Kosuri (Addgene plasmid # 129789 ; http://n2t.net/addgene:129789 ; RRID:Addgene_129789)

  • For your References section:

    Structural and functional characterization of G protein-coupled receptors with deep mutational scanning. Jones EM, Lubock NB, Venkatakrishnan AJ, Wang J, Tseng AM, Paggi JM, Latorraca NR, Cancilla D, Satyadi M, Davis JE, Babu MM, Dror RO, Kosuri S. Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895. 10.7554/eLife.54895 PubMed 33084570
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