Bxb1Donor_W99A_ADRB2_LucReporter
(Plasmid
#129789)
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Purposeencodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGenewiz
- Backbone size w/o insert (bp) 6350
- Total vector size (bp) 7660
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameADRB2
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Alt nameBeta-2 Adrenergic Receptor
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SpeciesH. sapiens (human)
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Insert Size (bp)1314
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MutationW99A
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GenBank ID154
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Entrez GeneADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
- Promoter N/A
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttgtcgacgacggcggtct
- 3′ sequencing primer GCACACCGGCCTTATTCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bxb1Donor_W99A_ADRB2_LucReporter was a gift from Sriram Kosuri (Addgene plasmid # 129789 ; http://n2t.net/addgene:129789 ; RRID:Addgene_129789) -
For your References section:
Structural and functional characterization of G protein-coupled receptors with deep mutational scanning. Jones EM, Lubock NB, Venkatakrishnan AJ, Wang J, Tseng AM, Paggi JM, Latorraca NR, Cancilla D, Satyadi M, Davis JE, Babu MM, Dror RO, Kosuri S. Elife. 2020 Oct 21;9. pii: 54895. doi: 10.7554/eLife.54895. 10.7554/eLife.54895 PubMed 33084570