Unc104_K560GFP
(Plasmid #129773)

• Purpose: Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
• Depositing Lab: William Hancock
• Publication: Hancock Lab plasmids (unpublished)

Backbone

• Vector backbone: pET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing lab uses BL21(DE3) for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Unc104
• Alt name: kinesin-3
• Alt name: KIF1A
• Species: C. elegans (nematode)
• Mutation: Truncation at 361, fused to kinesin-1 starting Ala345
• Entrez Gene: unc-104 (a.k.a. CELE_C52E12.2)
• Tag / Fusion Protein:
  • GFP and His6 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry