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Addgene

KSP_HNL
(Plasmid #129772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab uses BL21(DE3) for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    KSP
  • Alt name
    kinesin-5
  • Alt name
    Eg5
  • Species
    X. laevis (frog)
  • Mutation
    Truncation at 367
  • Entrez Gene
    kif11.L (a.k.a. XELAEV_18034598mg, Cos2, Costal2, XLEg5K2, eg5, hksp, kif11, kif11-a, kif11-b, kif11a, knsl1, trip5)
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A D6E mutation in KSP is present but is not expected to impact the function of the protein. Requesting scientists should independently validate expected functionality.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KSP_HNL was a gift from William Hancock (Addgene plasmid # 129772 ; http://n2t.net/addgene:129772 ; RRID:Addgene_129772)
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