KIF3A560GFP
(Plasmid
#129769)
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PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDepositing lab uses BL21(DE3) for protein expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameKIF3A
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Alt namekinesin-2
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SpeciesM. musculus (mouse)
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MutationTruncation at 359, fused to kinesin-1 starting Ala345
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Entrez GeneKif3a (a.k.a. Kif3, Kifl, Kns3)
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Tag
/ Fusion Protein
- GFP and His6 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KIF3A560GFP was a gift from William Hancock (Addgene plasmid # 129769 ; http://n2t.net/addgene:129769 ; RRID:Addgene_129769)