K560_AviN_coBirA
(Plasmid #129766)

• Purpose: Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
• Depositing Lab: William Hancock
• Publication: Hancock Lab plasmids (unpublished)

Backbone

• Vector backbone: pET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing lab uses BL21(DE3) for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: KHC
• Alt name: kinesin-1
• Species: D. melanogaster (fly)
• Mutation: Truncation at 559
• Entrez Gene: Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
• Tags / Fusion Proteins:
  • Avi-tag GLNDIFEAQKIEWH (N terminal on insert)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Numbering differs compared to NCBI entry for Khc (NM_057242.5) in that truncation follows residue 557. This difference does not impact the function of the plasmid.