pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG
(Plasmid
#129758)
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PurposeDox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129758 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 9002
- Total vector size (bp) 9874
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORF18
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SpeciesKaposi's sarcoma-associated herpesvirus (HHV-8)
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Insert Size (bp)882
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Mutationchanged Glutamic acid 36 to Alanine and Leucine 37 to Alanine
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Entrez GeneORF18 (a.k.a. HHV8GK18_gp22)
- Promoter TRE3Gs
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer gggcgcctataaaagagtgctg
- 3′ sequencing primer ctctgacggccctcctgt (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG was a gift from Britt Glaunsinger (Addgene plasmid # 129758 ; http://n2t.net/addgene:129758 ; RRID:Addgene_129758) -
For your References section:
The interaction between ORF18 and ORF30 is required for late gene expression in Kaposi's sarcoma-associated herpesvirus. Castaneda AF, Glaunsinger BA. J Virol. 2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18. 10.1128/JVI.01488-18 PubMed 30305361