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Addgene

pRK5-myc-Cdc42-Q61L
(Plasmid #12974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-Myc
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc42 constitutively active
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    576
  • Mutation
    Q61L Constitutively active
  • GenBank ID
    M57298
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-Cdc42-Q61L was a gift from Gary Bokoch (Addgene plasmid # 12974 ; http://n2t.net/addgene:12974 ; RRID:Addgene_12974)
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