Skylan-S-N1
(Plasmid
#129737)
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Purpose(Empty Backbone) A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- Skylan-S (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Skylan was generated by mutating H62S of mEos3.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Skylan-S-N1 was a gift from Pingyong Xu (Addgene plasmid # 129737 ; http://n2t.net/addgene:129737 ; RRID:Addgene_129737) -
For your References section:
Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI). Zhang X, Chen X, Zeng Z, Zhang M, Sun Y, Xi P, Peng J, Xu P. ACS Nano. 2015 Mar 24;9(3):2659-67. doi: 10.1021/nn5064387. Epub 2015 Feb 23. 10.1021/nn5064387 PubMed 25695314