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Addgene

pENO1-iRFP-NATr
(Plasmid #129731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-simple
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 6000
  • Modifications to backbone
    None
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iRFP-Candida albicans codon-optimized
  • Promoter ENO1 from Candida albicans

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer not available
  • 3′ sequencing primer not available
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENO1-iRFP-NATr was a gift from Robert Wheeler (Addgene plasmid # 129731 ; http://n2t.net/addgene:129731 ; RRID:Addgene_129731)

  • For your References section:

    Candida albicans and Pseudomonas aeruginosa Interact To Enhance Virulence of Mucosal Infection in Transparent Zebrafish. Bergeron AC, Seman BG, Hammond JH, Archambault LS, Hogan DA, Wheeler RT. Infect Immun. 2017 Oct 18;85(11). pii: IAI.00475-17. doi: 10.1128/IAI.00475-17. Print 2017 Nov. 10.1128/IAI.00475-17 PubMed 28847848
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