Skip to main content
Addgene

pMS22
(Plasmid #129710)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSY5
  • Backbone manufacturer
    lab made
  • Backbone size w/o insert (bp) 5387
  • Total vector size (bp) 6365
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Escherichia coli RssB-GSGS variant
  • Species
    Escherichia coli MG1655
  • Insert Size (bp)
    1012
  • Mutation
    RssB E134G:E135S:E136G:E137S
  • GenBank ID
    AYG19683.1
  • Entrez Gene
    rssB (a.k.a. b1235, ECK1230, hnr, sprE, ychL)
  • Promoter T7
  • Tag / Fusion Protein
    • 10-Histidine tag cleavable with PreScission Protease (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7p
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was amplified by PCR from plasmid pT7-RssB whose construction was reported by the Gottesman lab in Battesti A, Hoskins JR, Tong S, Milanesio P, Mann JM, Kravats A, Tsegaye YM, Bougdour A, Wickner S, Gottesman S. 2013. Anti-adaptors provide multiple modes for regulation of the RssB adaptor protein. Genes Dev 27: 2722–2735.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS22 was a gift from Alexandra Deaconescu (Addgene plasmid # 129710 ; http://n2t.net/addgene:129710 ; RRID:Addgene_129710)

  • For your References section:

    Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor. Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM. Genes Dev. 2019 Jun 1;33(11-12):718-732. doi: 10.1101/gad.320168.118. Epub 2019 Apr 11. 10.1101/gad.320168.118 PubMed 30975721
Related items: