Twinkle-APEX2-V5
(Plasmid #129705)

• Purpose: APEX2 fused to the mitochondrial nucleoid protein Twinkle
• Depositing Lab: Alice Ting
• Publication: Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-414. doi: 10.1016/j.chembiol.2017.02.002. Epub 2017 Feb 23.

Backbone

• Vector backbone: pLX304
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Twinkle-APEX2-V5
• Species: H. sapiens (human)
• Entrez Gene: TWNK (a.k.a. ATXN8, C10orf2, IOSCA, MTDPS7, PEO, PEO1, PEOA3, PRLTS5, SANDO, SCA8, TWINL)
• Entrez Gene: APEX2 (a.k.a. APE2, APEXL2, XTH2, ZGRF2)
• Promoter: CMV promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: WPRE-reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry