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Addgene

pET15b-GreBdm-D41N
(Plasmid #129691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JC10289
  • Growth instructions
    The depositing lab recommends using a strain expressing T7 RNA polymerase, like BL21(DE3), for expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GreB E82C C68S D41N
  • Species
    E. coli
  • Entrez Gene
    greB (a.k.a. b3406, ECK3393)
  • Tag / Fusion Protein
    • his6 (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-GreBdm-D41N was a gift from Jeff Gelles (Addgene plasmid # 129691 ; http://n2t.net/addgene:129691 ; RRID:Addgene_129691)

  • For your References section:

    Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue. Tetone LE, Friedman LJ, Osborne ML, Ravi H, Kyzer S, Stumper SK, Mooney RA, Landick R, Gelles J. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1081-E1090. doi: 10.1073/pnas.1616525114. Epub 2017 Jan 30. 10.1073/pnas.1616525114 PubMed 28137878
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