pcDNA3-EGFP-RhoA-Q63L
(Plasmid #12968)

• Depositing Lab: Gary Bokoch
• Publication: Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-33.

Backbone

• Vector backbone: pcDNA3-EGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoA constitutively active
• Alt name: RhoA
• Species: H. sapiens (human)
• Insert Size (bp): 582
• Mutation: Q63L Constitutively active
• GenBank ID: L25080
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 for EGFP
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 38 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry