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Addgene

pYPQ291-D573A
(Plasmid #129676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Total vector size (bp) 6579
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible BthCas12b entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BthCas12b
  • Species
    Bacillus thermoamylovorans
  • Mutation
    D573A; BthCas12b is rice codon optimized

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ291-D573A was a gift from Yiping Qi (Addgene plasmid # 129676 ; http://n2t.net/addgene:129676 ; RRID:Addgene_129676)

  • For your References section:

    CRISPR-Cas12b enables efficient plant genome engineering. Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, Qi Y. Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9. 10.1038/s41477-020-0614-6 PubMed 32170285
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