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Purpose(Empty Backbone) Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-EF1a-DIO-mCherry
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Backbone manufacturerKarl Deisseroth
- Backbone size (bp) 5941
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Vector typeAAV
- Promoter EF1a
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Tag
/ Fusion Protein
- mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The construct holds a sequence containing the distal (DSE) and proximal elements (PSE) of the U6 promoter spaced by the CDS to express shRNAs
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDIO-DSE-mCherry-PSE-MCS was a gift from Beatriz Rico (Addgene plasmid # 129669 ; http://n2t.net/addgene:129669 ; RRID:Addgene_129669) -
For your References section:
Distinct molecular programs regulate synapse specificity in cortical inhibitory circuits. Favuzzi E, Deogracias R, Marques-Smith A, Maeso P, Jezequel J, Exposito-Alonso D, Balia M, Kroon T, Hinojosa AJ, F Maraver E, Rico B. Science. 2019 Jan 25;363(6425):413-417. doi: 10.1126/science.aau8977. 10.1126/science.aau8977 PubMed 30679375