pDIO-DSE-mCherry-PSE-MCS
(Plasmid #129669)

• Purpose: (Empty Backbone) Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed
• Depositing Lab: Beatriz Rico
• Publication: Favuzzi et al Science. 2019 Jan 25;363(6425):413-417. doi: 10.1126/science.aau8977.

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Backbone

• Vector backbone: pAAV-EF1a-DIO-mCherry
• Backbone manufacturer: Karl Deisseroth
• Backbone size (bp): 5941
• Vector type: AAV
• Promoter: EF1a
• Tag / Fusion Protein:
  • mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF1a-F

Resource Information

• Supplemental Documents:
  • pDIO-DSE-mCherry-PSE-MCS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct holds a sequence containing the distal (DSE) and proximal elements (PSE) of the U6 promoter spaced by the CDS to express shRNAs