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Addgene

pDIO-DSE-mCherry-PSE-MCS
(Plasmid #129669)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129669 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-EF1a-DIO-mCherry
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size (bp) 5941
  • Vector type
    AAV
  • Promoter EF1a
  • Tag / Fusion Protein
    • mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The construct holds a sequence containing the distal (DSE) and proximal elements (PSE) of the U6 promoter spaced by the CDS to express shRNAs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDIO-DSE-mCherry-PSE-MCS was a gift from Beatriz Rico (Addgene plasmid # 129669 ; http://n2t.net/addgene:129669 ; RRID:Addgene_129669)
  • For your References section:

    Distinct molecular programs regulate synapse specificity in cortical inhibitory circuits. Favuzzi E, Deogracias R, Marques-Smith A, Maeso P, Jezequel J, Exposito-Alonso D, Balia M, Kroon T, Hinojosa AJ, F Maraver E, Rico B. Science. 2019 Jan 25;363(6425):413-417. doi: 10.1126/science.aau8977. 10.1126/science.aau8977 PubMed 30679375