pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)
(Plasmid #129668)

• Purpose: A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus
• Depositing Lab: Michael Guertin
• Publication: Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-1455. doi: 10.1101/gad.328237.119. Epub 2019 Aug 29.

Backbone

• Vector backbone: pMK232
• Backbone manufacturer: Masato Kanemaki (Addgene plasmid # 72834)
• Backbone size w/o insert (bp): 9590
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsARF16-PB1 domain
• Species: Oryza sativa
• Insert Size (bp): 1377
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has GFP-ARF16-PB1 in translation fusion with OsTIR1.

Please visit https://www.biorxiv.org/content/10.1101/585927v2 for bioRxiv preprint.