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Addgene

JME5000
(Plasmid #129661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1A3
  • Backbone size (bp) 2000
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    EYK1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JME5000 was a gift from Tristan Rossignol (Addgene plasmid # 129661 ; http://n2t.net/addgene:129661 ; RRID:Addgene_129661)

  • For your References section:

    A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity. Larroude M, Trabelsi H, Nicaud JM, Rossignol T. Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4. 10.1007/s10529-020-02805-4 PubMed 31974649
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