JME4390
(Plasmid
#129656)
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Purpose(Empty Backbone) LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB1A3
- Backbone size (bp) 2000
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Vector typeYeast Expression, CRISPR
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JME4390 was a gift from Tristan Rossignol (Addgene plasmid # 129656 ; http://n2t.net/addgene:129656 ; RRID:Addgene_129656) -
For your References section:
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity. Larroude M, Trabelsi H, Nicaud JM, Rossignol T. Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4. 10.1007/s10529-020-02805-4 PubMed 31974649