pT2ADWpuro_2paRAF
(Plasmid #129653)

• Purpose: For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1
• Depositing Lab: Michiyuki Matsuda
• Publication: Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 10.1038/s41592-019-0541-5.

Backbone

• Vector backbone: pT2ADWpuro
• Backbone size w/o insert (bp): 8500
• Modifications to backbone: pT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
• Species: H. sapiens (human)
• Insert Size (bp): 5500
• Promoter: CAG

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pCAGGS-5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry