pCAGGS_2paCRY2(D387A)
(Plasmid
#129652)
-
Purpose2P-activatable CRY2 with the FAD-disrupting mutation
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAGGS
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert name2paCRY2(D387A)
-
SpeciesA. thaliana (mustard weed)
-
Insert Size (bp)2100
- Promoter CAG
-
Tag
/ Fusion Protein
- mTagBFP2
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCAGGS-5 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAGGS_2paCRY2(D387A) was a gift from Michiyuki Matsuda (Addgene plasmid # 129652 ; http://n2t.net/addgene:129652 ; RRID:Addgene_129652) -
For your References section:
FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M. Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 10.1038/s41592-019-0541-5. 10.1038/s41592-019-0541-5 PubMed 31501546
