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Addgene

pHis APEX2-eIF4A1
(Plasmid #129645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eukaryotic translation initiation factor 4A1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001416.4
  • Entrez Gene
    EIF4A1 (a.k.a. DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I)
  • Tags / Fusion Proteins
    • APEX2 (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis APEX2-eIF4A1 was a gift from Nicholas Ingolia (Addgene plasmid # 129645 ; http://n2t.net/addgene:129645 ; RRID:Addgene_129645)

  • For your References section:

    Proximity RNA Labeling by APEX-Seq Reveals the Organization of Translation Initiation Complexes and Repressive RNA Granules. Padron A, Iwasaki S, Ingolia NT. Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 10.1016/j.molcel.2019.07.030. 10.1016/j.molcel.2019.07.030 PubMed 31442426
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