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Addgene

pcDNA5/FRT/TO APEX2-eIF4E1
(Plasmid #129644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6509
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EIF4E1
  • Species
    H. sapiens (human)
  • Entrez Gene
    EIF4E (a.k.a. AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E)
  • Tag / Fusion Protein
    • APEX2 (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO APEX2-eIF4E1 was a gift from Nicholas Ingolia (Addgene plasmid # 129644 ; http://n2t.net/addgene:129644 ; RRID:Addgene_129644)

  • For your References section:

    Proximity RNA Labeling by APEX-Seq Reveals the Organization of Translation Initiation Complexes and Repressive RNA Granules. Padron A, Iwasaki S, Ingolia NT. Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 10.1016/j.molcel.2019.07.030. 10.1016/j.molcel.2019.07.030 PubMed 31442426
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