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Addgene

mNeongreen-pGBD(PKN1 codon optimized)
(Plasmid #129634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RhoA binding doamin of PKN1(30 - 100) codon optimized
  • Species
    Synthetic
  • Promoter CMVdel

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNeongreen-pGBD(PKN1 codon optimized) was a gift from Dorus Gadella (Addgene plasmid # 129634 ; http://n2t.net/addgene:129634 ; RRID:Addgene_129634)

  • For your References section:

    Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J. J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. 10.1242/jcs.258823 PubMed 34357388
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