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PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
(Plasmid #129632)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129632 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG was a gift from Dorus Gadella (Addgene plasmid # 129632 ; http://n2t.net/addgene:129632 ; RRID:Addgene_129632)

  • For your References section:

    Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors. Chavez-Abiega S, Gronloh MLB, Gadella TWJ, Bruggeman FJ, Goedhart J. J Cell Sci. 2022 Mar 15;135(6):jcs259685. doi: 10.1242/jcs.259685. Epub 2022 Mar 11. 10.1242/jcs.259685 PubMed 35107584
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