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Addgene

mCherry-ITSN2
(Plasmid #129615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ITSN2
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains N724D, M1499V, and I1857V mutations in the mCherry-ITSN2 insert. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-ITSN2 was a gift from Dorus Gadella (Addgene plasmid # 129615 ; http://n2t.net/addgene:129615 ; RRID:Addgene_129615)

  • For your References section:

    Identification of guanine nucleotide exchange factors that increase Cdc42 activity in primary human endothelial cells. Reinhard NR, Van Der Niet S, Chertkova A, Postma M, Hordijk PL, Gadella TWJ Jr, Goedhart J. Small GTPases. 2019 Aug 30:1-15. doi: 10.1080/21541248.2019.1658509. 10.1080/21541248.2019.1658509 PubMed 31469028
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