Skip to main content
Addgene

Human INPP5A
(Plasmid #129587)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMAL-cRI
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6133
  • Total vector size (bp) 7997
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Type I inositol 5-phosphatase
  • Alt name
    INPP5A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    X77567.1
  • Entrez Gene
    INPP5A (a.k.a. 5PTASE)
  • Tag / Fusion Protein
    • Maltose binding protein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer classical primes of pMAL-cRI vector
  • 3′ sequencing primer classical primer of pMAL-cRI vector
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human INPP5A was a gift from Christophe Erneux (Addgene plasmid # 129587 ; http://n2t.net/addgene:129587 ; RRID:Addgene_129587)

  • For your References section:

    Production of recombinant human brain type I inositol-1,4,5-trisphosphate 5-phosphatase in Escherichia coli. Lack of phosphorylation by protein kinase C. Erneux C, De Smedt F, Moreau C, Rider M, Communi D. Eur J Biochem. 1995 Dec 1;234(2):598-602. doi: 10.1111/j.1432-1033.1995.598_b.x. 10.1111/j.1432-1033.1995.598_b.x PubMed 8536709
Related items: