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pET-29b-3xVSVG-GSK3(1-97)
(Plasmid #129583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET 29b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 5808
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GSK3a(1-97)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    438
  • GenBank ID
    NM_019884
  • Entrez Gene
    GSK3A
  • Tag / Fusion Protein
    • 6xHistidine (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor uses BL21 for protein production.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-29b-3xVSVG-GSK3(1-97) was a gift from Kevin Janes (Addgene plasmid # 129583 ; http://n2t.net/addgene:129583 ; RRID:Addgene_129583)

  • For your References section:

    An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650
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