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pGEX-4T-1-3xVSVG-GSK3(1-97)
(Plasmid #129582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GSK3a(1-97)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • GenBank ID
    NM_019884
  • Entrez Gene
    GSK3A
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • VSVG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5GEX
  • 3′ sequencing primer 3GEX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor uses BL21 for protein production.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-3xVSVG-GSK3(1-97) was a gift from Kevin Janes (Addgene plasmid # 129582 ; http://n2t.net/addgene:129582 ; RRID:Addgene_129582)

  • For your References section:

    An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650
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