pGEX-4T-1-3xFLAG
(Plasmid
#129570)
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Purpose(Empty Backbone) Empty backbone for FLAG tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T1
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Backbone manufacturerAmersham
- Backbone size (bp) 4969
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Modifications to backbone3xFLAG tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFLAG, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5GEX
- 3′ sequencing primer 3GEX (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor uses BL21 for protein production.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1-3xFLAG was a gift from Kevin Janes (Addgene plasmid # 129570 ; http://n2t.net/addgene:129570 ; RRID:Addgene_129570) -
For your References section:
An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650
