Clim-DC2-NMM-tdtomato
(Plasmid
#129557)
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PurposepClimDC-NMM:tdT cell membrane marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcr2.1 without kanamycin resistance gene
- Backbone size w/o insert (bp) 4500
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Vector typeBacterial Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-Myristoylation motif (NMM) fused to tdtomato
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Alt namepClimDC-NMM:tdT
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SpeciesCorallochytrium limacisporum
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Insert Size (bp)1479
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Clim-DC2-NMM-tdtomato was a gift from Inaki Ruiz-Trillo (Addgene plasmid # 129557 ; http://n2t.net/addgene:129557 ; RRID:Addgene_129557) -
For your References section:
Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives. Kozyczkowska A, Najle SR, Ocana-Pallares E, Areste C, Shabardina V, Ara PS, Ruiz-Trillo I, Casacuberta E. Curr Biol. 2021 Sep 27;31(18):4104-4110.e5. doi: 10.1016/j.cub.2021.06.061. Epub 2021 Jul 21. 10.1016/j.cub.2021.06.061 PubMed 34293333