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Addgene

Clim-DC2-NMM-tdtomato
(Plasmid #129557)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129557 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcr2.1 without kanamycin resistance gene
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-Myristoylation motif (NMM) fused to tdtomato
  • Alt name
    pClimDC-NMM:tdT
  • Species
    Corallochytrium limacisporum
  • Insert Size (bp)
    1479

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Clim-DC2-NMM-tdtomato was a gift from Inaki Ruiz-Trillo (Addgene plasmid # 129557 ; http://n2t.net/addgene:129557 ; RRID:Addgene_129557)
  • For your References section:

    Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives. Kozyczkowska A, Najle SR, Ocana-Pallares E, Areste C, Shabardina V, Ara PS, Ruiz-Trillo I, Casacuberta E. Curr Biol. 2021 Sep 27;31(18):4104-4110.e5. doi: 10.1016/j.cub.2021.06.061. Epub 2021 Jul 21. 10.1016/j.cub.2021.06.061 PubMed 34293333