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Addgene

Clim-DC2-H2B2-Venus
(Plasmid #129556)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129556 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcr2.1 without kanamycin resistance gene
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone 2B fused to venus
  • Alt name
    pClimDC-H2B:mV
  • Species
    Corallochytrium limacisporum
  • Insert Size (bp)
    1122

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Clim-DC2-H2B2-Venus was a gift from Inaki Ruiz-Trillo (Addgene plasmid # 129556 ; http://n2t.net/addgene:129556 ; RRID:Addgene_129556)
  • For your References section:

    Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives. Kozyczkowska A, Najle SR, Ocana-Pallares E, Areste C, Shabardina V, Ara PS, Ruiz-Trillo I, Casacuberta E. Curr Biol. 2021 Sep 27;31(18):4104-4110.e5. doi: 10.1016/j.cub.2021.06.061. Epub 2021 Jul 21. 10.1016/j.cub.2021.06.061 PubMed 34293333