pCRISPomyces-SaCas9
(Plasmid
#129553)
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PurposeContains codon-optimized SaCas9 and gRNA for streptomyces
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKC1139
- Total vector size (bp) 10217
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Modifications to backbonemutation G8694C to remove BbsI site from pSG5 rep
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSaCas9
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Alt nameStaphylococcus aureus Cas9
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Insert Size (bp)3158
- Promoter rpsL(XC)-BbsI
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRISPomyces-SaCas9 was a gift from Fong Tian Wong & Mingzi M. Zhang (Addgene plasmid # 129553 ; http://n2t.net/addgene:129553 ; RRID:Addgene_129553) -
For your References section:
Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes. Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, Wong FT. Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021. 10.1002/bit.27021 PubMed 31090220