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Addgene

pCRISPomyces-Sth1Cas9
(Plasmid #129552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKC1139
  • Total vector size (bp) 10417
  • Modifications to backbone
    mutation G8694C to remove BbsI site from pSG5 rep
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sth1Cas9
  • Alt name
    Streptococcus thermophilus CRISPR1
  • Insert Size (bp)
    3363
  • Promoter rpsL(XC)-BbsI

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-Sth1Cas9 was a gift from Fong Tian Wong & Mingzi M. Zhang (Addgene plasmid # 129552 ; http://n2t.net/addgene:129552 ; RRID:Addgene_129552)

  • For your References section:

    Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes. Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, Wong FT. Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021. 10.1002/bit.27021 PubMed 31090220
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