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pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)
(Plasmid #129539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ151 addgene plasmid #19330
  • Vector type
    Worm Expression, Cre/Lox ; mos1-mediated Single Copy Insertion (mosSCI)
  • Selectable markers
    C. br. unc-119(+)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC NGS identified a single nucleotide discrepancy in the unc-119 promoter. The depositing laboratory has confirmed that this should not disrupt plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP) was a gift from Arneet Saltzman (Addgene plasmid # 129539 ; http://n2t.net/addgene:129539 ; RRID:Addgene_129539)

  • For your References section:

    Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes. Aram R, MacGillivray K, Li C, Saltzman A. MicroPubl Biol. 2019 Aug 27;2019. doi: 10.17912/micropub.biology.000146. 10.17912/micropub.biology.000146 PubMed 32550440
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