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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
(Plasmid #129537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57_PB-PGK-tetR-KRAB-2A-Puro
  • Backbone size w/o insert (bp) 5177
  • Total vector size (bp) 5518
  • Vector type
    Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetR-3xFlag-HA-2A-Puro
  • Species
    Synthetic
  • Insert Size (bp)
    1539
  • Promoter hPGK promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (not destroyed)
  • 3′ cloning site BstbI (not destroyed)
  • 5′ sequencing primer TCGAGGATCAGGAACATCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro was a gift from Hideyuki Okano (Addgene plasmid # 129537 ; http://n2t.net/addgene:129537 ; RRID:Addgene_129537)

  • For your References section:

    Controlling gene activation by enhancers through a drug-inducible topological insulator. Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Hoshi K, Takato T, Toyoda A, Okano H, Hishikawa K. Elife. 2020 May 5;9. pii: 47980. doi: 10.7554/eLife.47980. 10.7554/eLife.47980 PubMed 32369019
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