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Addgene

pUC-STITCH
(Plasmid #129535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCIDT
  • Backbone manufacturer
    Integrated DNA technologies
  • Backbone size w/o insert (bp) 2764
  • Total vector size (bp) 5749
  • Vector type
    CRISPR ; donor template
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STITCH
  • Alt name
    Synthetic Topological Insulator with TetO for Chromatin-Engineering
  • Species
    Synthetic
  • Insert Size (bp)
    2985

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-STITCH was a gift from Hideyuki Okano (Addgene plasmid # 129535 ; http://n2t.net/addgene:129535 ; RRID:Addgene_129535)

  • For your References section:

    Controlling gene activation by enhancers through a drug-inducible topological insulator. Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Hoshi K, Takato T, Toyoda A, Okano H, Hishikawa K. Elife. 2020 May 5;9. pii: 47980. doi: 10.7554/eLife.47980. 10.7554/eLife.47980 PubMed 32369019
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