p1195-pssAAV.U1a.hNme2Cas9
(Plasmid #129534)

• Purpose: AAV vector expressing Nme2Cas9
• Depositing Lab: Erik Sontheimer
• Publication: Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 3326
• Total vector size (bp): 6833
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human codon-optimized Nme2Cas9
• Alt name: Nme2Cas9c
• Promoter: U1a
• Tags / Fusion Proteins:
  • 2xNLS (N terminal on insert)
  • NLS-3xHA-NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: cgtggccacgcaactcatac
• 3′ sequencing primer: ccgccgagtgagagacacaa

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry