DCT.VV.Orf265
(Plasmid #12953)

• Purpose: This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
• Depositing Labs: Jack Bennink, Jon Yewdell
• Publication: Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–104.

Backbone

• Vector backbone: pcDNA 3.1D V5-His-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5514
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VV.Orf265
• Species: Vaccinia Virus
• Insert Size (bp): 387

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Universal T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.