pSL1166
(Plasmid #129520)

• Purpose: pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation
• Depositing Lab: Scott Lindner
• Publication: Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-9566. doi: 10.1074/jbc.RA118.007121. Epub 2019 May 1.

Backbone

• Vector backbone: pDEF
• Vector type: Plasmodium, rodent-infectious species
• Selectable markers: Pyrimethamine

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9
• Species: S. pyogenes
• Insert Size (bp): 4269
• Tag / Fusion Protein:
  • 3XFLAG-NLS (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pSL1166.gb
• A portion of this plasmid was derived from a plasmid made by: Gene origin for SpCas9 is from Addgene #48138

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there was a 20bp deletion identified between the depositor's Genbank sequence and Addgene's quality control sequence. The depositor noted that this deletion does NOT affect plasmid function.