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pLPC-Flag-SOCS1-Y80D
(Plasmid #129516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6134
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOCS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    660
  • Mutation
    Changed Tyrosine 80 to Aspartic acid
  • GenBank ID
    NM_003745
  • Entrez Gene
    SOCS1 (a.k.a. AISIMD, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP-3, TIP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer taggcgtgtacggtggga
  • 3′ sequencing primer cttgccaaacctacaggt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-Flag-SOCS1-Y80D was a gift from Gerardo Ferbeyre (Addgene plasmid # 129516 ; http://n2t.net/addgene:129516 ; RRID:Addgene_129516)

  • For your References section:

    Phosphorylation of SOCS1 Inhibits the SOCS1-p53 Tumor Suppressor Axis. Saint-Germain E, Mignacca L, Huot G, Acevedo M, Moineau-Vallee K, Calabrese V, Bourdeau V, Rowell MC, Ilangumaran S, Lessard F, Ferbeyre G. Cancer Res. 2019 Jul 1;79(13):3306-3319. doi: 10.1158/0008-5472.CAN-18-1503. Epub 2019 May 17. 10.1158/0008-5472.CAN-18-1503 PubMed 31101761
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