pLPC-Flag-SOCS1
(Plasmid #129514)

• Purpose: Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal
• Depositing Lab: Gerardo Ferbeyre
• Publication: Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319. doi: 10.1158/0008-5472.CAN-18-1503. Epub 2019 May 17.

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6134
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOCS1
• Species: H. sapiens (human)
• Insert Size (bp): 660
• GenBank ID: NM_003745
• Entrez Gene: SOCS1 (a.k.a. AISIMD, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP-3, TIP3)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: taggcgtgtacggtggga
• 3′ sequencing primer: cttgccaaacctacaggt

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry